pAAV-G-hSyn-Blank Amp

1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt 61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact 121 aggggttcct gcggccgccc taggctagac tgcagagggc cctgcgtatg agtgcaagtg 181 ggttttagga ccaggatgag gcggggtggg ggtgcctacc tgacgaccga ccccgaccca 241 ctggacaagc acccaacccc cattccccaa attgcgcatc ccctatcaga gagggggagg 301 ggaaacagga tgcggcgagg cgcgtgcgca ctgccagctt cagcaccgcg gacagtgcct 361 tcgcccccgc ctggcggcgc gcgccaccgc cgcctcagca ctgaaggcgc gctgacgtca 421 ctcgccggtc ccccgcaaac tccccttccc ggccaccttg gtcgcgtccg cgccgccgcc 481 ggcccagccg gaccgcacca cgcgaggcgc gagatagggg ggcacgggcg cgaccatctg 541 cgctgcggcg ccggcgactc agcgctgcct cagtctgcgg tgggcagcgg aggagtcgtg 601 tcgtgcctga gagcgcagtc gagaagagct cgtttagtga accgtcagat cgcctggaga 661 cgccatccac gctgttttga cctccataga agaaccgagt ttaaactccc tatcagtgat 721 agagatctcc ctatcagtga tagagagcta gcgatatctg actcgagcca atcttaaggg 781 gtggcatccc tgtgacccct ccccagtgcc tctcctggcc ctggaagttg ccactccagt 841 gcccaccagc cttgtcctaa taaaattaag ttgcatcatt ttgtctgact aggtgtcctt 901 ctataatatt atggggtgga ggggggtggt atggagcaag gggcaagttg ggaagacaac 961 ctgtagggcc tgcggggtct attgggaacc aagctggagt gcagtggcac aatcttggct 1021 cactgcaatc tccgcctcct gggttcaagc gattctcctg cctcagcctc ccgagttgtt 1081 gggattccag gcatgcatga ccaggctcag ctaatttttg tttttttggt agagacgggg 1141 tttcaccata ttggccaggc tggtctccaa ctcctaatct caggtgatct acccaccttg 1201 gcctcccaaa ttgctgggat tacaggcgtg aaccactgct cccttccctg tccttcacgt 1261 gcggaccgag cggccgcagg aacccctagt gatggagttg gccactccct ctctgcgcgc 1321 tcgctcgctc actgaggccg ggcgaccaaa ggtcgcccga cgcccgggct ttgcccgggc 1381 ggcctcagtg agcgagcgag cgcgcagctg cctgcagggg cgcctgatgc ggtattttct 1441 ccttacgcat ctgtgcggta tttcacaccg catacgtcaa agcaaccata gtacgcgccc 1501 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt 1561 gccagcgcct tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc 1621 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta 1681 cggcacctcg accccaaaaa acttgatttg ggtgatggtt cacgtagtgg gccatcgccc 1741 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg 1801 ttccaaactg gaacaacact caactctatc tcgggctatt cttttgattt ataagggatt 1861 ttgccgattt cggtctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat 1921 tttaacaaaa tattaacgtt tacaatttta tggtgcactc tcagtacaat ctgctctgat 1981 gccgcatagt taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct 2041 tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt 2101 cagaggtttt caccgtcatc accgaaacgc gcgagccggg agctgcatgt gtcagaggtt 2161 ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc tatttttata 2221 ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt 2281 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag 2341 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca 2401 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc 2461 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat 2521 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc 2581 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg 2641 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc 2701 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat 2761 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga 2821 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc 2881 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc 2941 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt 3001 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc 3061 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc 3121 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca 3181 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca 3241 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt 3301 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta 3361 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg 3421 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc 3481 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag 3541 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa 3601 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc 3661 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc 3721 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta 3781 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag 3841 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct 3901 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga 3961 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc 4021 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgt //

1 cctgcaggcagctgcgcgctcgctcgctcactgaggccgcccgggcgtcgggcgaccttt 60 GGACGTCCGTCGACGCGCGAGCGAGCGAGTGACTCCGGCGGGCCCGCAGCCCGCTGGAAA 61 ggtcgcccggcctcagtgagcgagcgagcgcgcagagagggagtggccaactccatcact 120 CCAGCGGGCCGGAGTCACTCGCTCGCTCGCGCGTCTCTCCCTCACCGGTTGAGGTAGTGA ApaI | 121 aggggttcctgcggccgcCCTAGGCTAGACTGCAGAGGGCCCTGCGTATGAGTGCAAGTG 180 TCCCCAAGGACGCCGGCGGGATCCGATCTGACGTCTCCCGGGACGCATACTCACGTTCAC 181 GGTTTTAGGACCAGGATGAGGCGGGGTGGGGGTGCCTACCTGACGACCGACCCCGACCCA 240 CCAAAATCCTGGTCCTACTCCGCCCCACCCCCACGGATGGACTGCTGGCTGGGGCTGGGT 241 CTGGACAAGCACCCAACCCCCATTCCCCAAATTGCGCATCCCCTATCAGAGAGGGGGAGG 300 GACCTGTTCGTGGGTTGGGGGTAAGGGGTTTAACGCGTAGGGGATAGTCTCTCCCCCTCC 301 GGAAACAGGATGCGGCGAGGCGCGTGCGCACTGCCAGCTTCAGCACCGCGGACAGTGCCT 360 CCTTTGTCCTACGCCGCTCCGCGCACGCGTGACGGTCGAAGTCGTGGCGCCTGTCACGGA 361 TCGCCCCCGCCTGGCGGCGCGCGCCACCGCCGCCTCAGCACTGAAGGCGCGCTGACGTCA 420 AGCGGGGGCGGACCGCCGCGCGCGGTGGCGGCGGAGTCGTGACTTCCGCGCGACTGCAGT 421 CTCGCCGGTCCCCCGCAAACTCCCCTTCCCGGCCACCTTGGTCGCGTCCGCGCCGCCGCC 480 GAGCGGCCAGGGGGCGTTTGAGGGGAAGGGCCGGTGGAACCAGCGCAGGCGCGGCGGCGG 481 GGCCCAGCCGGACCGCACCACGCGAGGCGCGAGATAGGGGGGCACGGGCGCGACCATCTG 540 CCGGGTCGGCCTGGCGTGGTGCGCTCCGCGCTCTATCCCCCCGTGCCCGCGCTGGTAGAC 541 CGCTGCGGCGCCGGCGACTCAGCGCTGCCTCAGTCTGCGGTGGGCAGCGGAGGAGTCGTG 600 GCGACGCCGCGGCCGCTGAGTCGCGACGGAGTCAGACGCCACCCGTCGCCTCCTCAGCAC SacI | 601 TCGTGCCTGAGAGCGCAGTCGAGAAgagctcgtttagtgaaccgtcagatcgcctggaga 660 AGCACGGACTCTCGCGTCAGCTCTTCTCGAGCAAATCACTTGGCAGTCTAGCGGACCTCT tetO reg(707,746)>>> | 661 cgccatccacgctgttttgacctccatagaagaaccgagtttaaactccctatcagtgat 720 GCGGTAGGTGCGACAAAACTGGAGGTATCTTCTTGGCTCAAATTTGAGGGATAGTCACTA BglII EcoRV XhoI hGH_PA term(779,1255)>>> | | | | 721 agagatctccctatcagtgatagagagctagcgatatctgactcgagccaatCTTAAGgg 780 TCTCTAGAGGGATAGTCACTATCTCTCGATCGCTATAGACTGAGCTCGGTTAGAATTCCC 781 gtggcatccctgtgacccctccccagtgcctctcctggccctggaagttgccactccagt 840 CACCGTAGGGACACTGGGGAGGGGTCACGGAGAGGACCGGGACCTTCAACGGTGAGGTCA 841 gcccaccagccttgtcctaataaaattaagttgcatcattttgtctgactaggtgtcctt 900 CGGGTGGTCGGAACAGGATTATTTTAATTCAACGTAGTAAAACAGACTGATCCACAGGAA 901 ctataatattatggggtggaggggggtggtatggagcaaggggcaagttgggaagacaac 960 GATATTATAATACCCCACCTCCCCCCACCATACCTCGTTCCCCGTTCAACCCTTCTGTTG 961 ctgtagggcctgcggggtctattgggaaccaagctggagtgcagtggcacaatcttggct 1020 GACATCCCGGACGCCCCAGATAACCCTTGGTTCGACCTCACGTCACCGTGTTAGAACCGA 1021 cactgcaatctccgcctcctgggttcaagcgattctcctgcctcagcctcccgagttgtt 1080 GTGACGTTAGAGGCGGAGGACCCAAGTTCGCTAAGAGGACGGAGTCGGAGGGCTCAACAA SphI | 1081 gggattccaggcatgcatgaccaggctcagctaatttttgtttttttggtagagacgggg 1140 CCCTAAGGTCCGTACGTACTGGTCCGAGTCGATTAAAAACAAAAAAACCATCTCTGCCCC 1141 tttcaccatattggccaggctggtctccaactcctaatctcaggtgatctacccaccttg 1200 AAAGTGGTATAACCGGTCCGACCAGAGGTTGAGGATTAGAGTCCACTAGATGGGTGGAAC 1201 gcctcccaaattgctgggattacaggcgtgaaccactgctcccttccctgtccttcacgt 1260 CGGAGGGTTTAACGACCCTAATGTCCGCACTTGGTGACGAGGGAAGGGACAGGAAGTGCA 1261 gcggaccgagcggccgcaggaacccctagtgatggagttggccactccctctctgcgcgc 1320 CGCCTGGCTCGCCGGCGTCCTTGGGGATCACTACCTCAACCGGTGAGGGAGAGACGCGCG 1321 tcgctcgctcactgaggccgggcgaccaaaggtcgcccgacgcccgggctttgcccgggc 1380 AGCGAGCGAGTGACTCCGGCCCGCTGGTTTCCAGCGGGCTGCGGGCCCGAAACGGGCCCG 1381 ggcctcagtgagcgagcgagcgcgcagctgcctgcaggggcgcctgatgcggtattttct 1440 CCGGAGTCACTCGCTCGCTCGCGCGTCGACGGACGTCCCCGCGGACTACGCCATAAAAGA 1441 ccttacgcatctgtgcggtatttcacaccgcatacgtcaaagcaaccatagtacgcgccc 1500 GGAATGCGTAGACACGCCATAAAGTGTGGCGTATGCAGTTTCGTTGGTATCATGCGCGGG f1 origin(1510,1816)>>> | 1501 tgtagcggcgcattaagcgcggcgggtgtggtggttacgcgcagcgtgaccgctacactt 1560 ACATCGCCGCGTAATTCGCGCCGCCCACACCACCAATGCGCGTCGCACTGGCGATGTGAA 1561 gccagcgccttagcgcccgctcctttcgctttcttcccttcctttctcgccacgttcgcc 1620 CGGTCGCGGAATCGCGGGCGAGGAAAGCGAAAGAAGGGAAGGAAAGAGCGGTGCAAGCGG 1621 ggctttccccgtcaagctctaaatcgggggctccctttagggttccgatttagtgcttta 1680 CCGAAAGGGGCAGTTCGAGATTTAGCCCCCGAGGGAAATCCCAAGGCTAAATCACGAAAT 1681 cggcacctcgaccccaaaaaacttgatttgggtgatggttcacgtagtgggccatcgccc 1740 GCCGTGGAGCTGGGGTTTTTTGAACTAAACCCACTACCAAGTGCATCACCCGGTAGCGGG 1741 tgatagacggtttttcgccctttgacgttggagtccacgttctttaatagtggactcttg 1800 ACTATCTGCCAAAAAGCGGGAAACTGCAACCTCAGGTGCAAGAAATTATCACCTGAGAAC 1801 ttccaaactggaacaacactcaactctatctcgggctattcttttgatttataagggatt 1860 AAGGTTTGACCTTGTTGTGAGTTGAGATAGAGCCCGATAAGAAAACTAAATATTCCCTAA 1861 ttgccgatttcggtctattggttaaaaaatgagctgatttaacaaaaatttaacgcgaat 1920 AACGGCTAAAGCCAGATAACCAATTTTTTACTCGACTAAATTGTTTTTAAATTGCGCTTA 1921 tttaacaaaatattaacgtttacaattttatggtgcactctcagtacaatctgctctgat 1980 AAATTGTTTTATAATTGCAAATGTTAAAATACCACGTGAGAGTCATGTTAGACGAGACTA 1981 gccgcatagttaagccagccccgacacccgccaacacccgctgacgcgccctgacgggct 2040 CGGCGTATCAATTCGGTCGGGGCTGTGGGCGGTTGTGGGCGACTGCGCGGGACTGCCCGA 2041 tgtctgctcccggcatccgcttacagacaagctgtgaccgtctccgggagctgcatgtgt 2100 ACAGACGAGGGCCGTAGGCGAATGTCTGTTCGACACTGGCAGAGGCCCTCGACGTACACA 2101 cagaggttttcaccgtcatcaccgaaacgcgcgagccgggagctgcatgtgtcagaggtt 2160 GTCTCCAAAAGTGGCAGTAGTGGCTTTGCGCGCTCGGCCCTCGACGTACACAGTCTCCAA 2161 ttcaccgtcatcaccgaaacgcgcgagacgaaagggcctcgtgatacgcctatttttata 2220 AAGTGGCAGTAGTGGCTTTGCGCGCTCTGCTTTCCCGGAGCACTATGCGGATAAAAATAT 2221 ggttaatgtcatgataataatggtttcttagacgtcaggtggcacttttcggggaaatgt 2280 CCAATTACAGTACTATTATTACCAAAGAATCTGCAGTCCACCGTGAAAAGCCCCTTTACA amp prom(2317,2345)>>> | 2281 gcgcggaacccctatttgtttatttttctaaatacattcaaatatgtatccgctcatgag 2340 CGCGCCTTGGGGATAAACAAATAAAAAGATTTATGTAAGTTTATACATAGGCGAGTACTC ORF_1 rf(2)(2387,3247)>>> | amp marker(2387,3247)>>> | 2341 acaataaccctgataaatgcttcaataatattgaaaaaggaagagtatgagtattcaaca 2400 TGTTATTGGGACTATTTACGAAGTTATTATAACTTTTTCCTTCTCATACTCATAAGTTGT 2401 tttccgtgtcgcccttattcccttttttgcggcattttgccttcctgtttttgctcaccc 2460 AAAGGCACAGCGGGAATAAGGGAAAAAACGCCGTAAAACGGAAGGACAAAAACGAGTGGG 2461 agaaacgctggtgaaagtaaaagatgctgaagatcagttgggtgcacgagtgggttacat 2520 TCTTTGCGACCACTTTCATTTTCTACGACTTCTAGTCAACCCACGTGCTCACCCAATGTA 2521 cgaactggatctcaacagcggtaagatccttgagagttttcgccccgaagaacgttttcc 2580 GCTTGACCTAGAGTTGTCGCCATTCTAGGAACTCTCAAAAGCGGGGCTTCTTGCAAAAGG 2581 aatgatgagcacttttaaagttctgctatgtggcgcggtattatcccgtattgacgccgg 2640 TTACTACTCGTGAAAATTTCAAGACGATACACCGCGCCATAATAGGGCATAACTGCGGCC 2641 gcaagagcaactcggtcgccgcatacactattctcagaatgacttggttgagtactcacc 2700 CGTTCTCGTTGAGCCAGCGGCGTATGTGATAAGAGTCTTACTGAACCAACTCATGAGTGG 2701 agtcacagaaaagcatcttacggatggcatgacagtaagagaattatgcagtgctgccat 2760 TCAGTGTCTTTTCGTAGAATGCCTACCGTACTGTCATTCTCTTAATACGTCACGACGGTA 2761 aaccatgagtgataacactgcggccaacttacttctgacaacgatcggaggaccgaagga 2820 TTGGTACTCACTATTGTGACGCCGGTTGAATGAAGACTGTTGCTAGCCTCCTGGCTTCCT 2821 gctaaccgcttttttgcacaacatgggggatcatgtaactcgccttgatcgttgggaacc 2880 CGATTGGCGAAAAAACGTGTTGTACCCCCTAGTACATTGAGCGGAACTAGCAACCCTTGG 2881 ggagctgaatgaagccataccaaacgacgagcgtgacaccacgatgcctgtagcaatggc 2940 CCTCGACTTACTTCGGTATGGTTTGCTGCTCGCACTGTGGTGCTACGGACATCGTTACCG 2941 aacaacgttgcgcaaactattaactggcgaactacttactctagcttcccggcaacaatt 3000 TTGTTGCAACGCGTTTGATAATTGACCGCTTGATGAATGAGATCGAAGGGCCGTTGTTAA 3001 aatagactggatggaggcggataaagttgcaggaccacttctgcgctcggcccttccggc 3060 TTATCTGACCTACCTCCGCCTATTTCAACGTCCTGGTGAAGACGCGAGCCGGGAAGGCCG 3061 tggctggtttattgctgataaatctggagccggtgagcgtgggtctcgcggtatcattgc 3120 ACCGACCAAATAACGACTATTTAGACCTCGGCCACTCGCACCCAGAGCGCCATAGTAACG 3121 agcactggggccagatggtaagccctcccgtatcgtagttatctacacgacggggagtca 3180 TCGTGACCCCGGTCTACCATTCGGGAGGGCATAGCATCAATAGATGTGCTGCCCCTCAGT 3181 ggcaactatggatgaacgaaatagacagatcgctgagataggtgcctcactgattaagca 3240 CCGTTGATACCTACTTGCTTTATCTGTCTAGCGACTCTATCCACGGAGTGACTAATTCGT 3241 ttggtaactgtcagaccaagtttactcatatatactttagattgatttaaaacttcattt 3300 AACCATTGACAGTCTGGTTCAAATGAGTATATATGAAATCTAACTAAATTTTGAAGTAAA 3301 ttaatttaaaaggatctaggtgaagatcctttttgataatctcatgaccaaaatccctta 3360 AATTAAATTTTCCTAGATCCACTTCTAGGAAAAACTATTAGAGTACTGGTTTTAGGGAAT pBR322 origin(3402,4021)>>> | 3361 acgtgagttttcgttccactgagcgtcagaccccgtagaaaagatcaaaggatcttcttg 3420 TGCACTCAAAAGCAAGGTGACTCGCAGTCTGGGGCATCTTTTCTAGTTTCCTAGAAGAAC 3421 agatcctttttttctgcgcgtaatctgctgcttgcaaacaaaaaaaccaccgctaccagc 3480 TCTAGGAAAAAAAGACGCGCATTAGACGACGAACGTTTGTTTTTTTGGTGGCGATGGTCG 3481 ggtggtttgtttgccggatcaagagctaccaactctttttccgaaggtaactggcttcag 3540 CCACCAAACAAACGGCCTAGTTCTCGATGGTTGAGAAAAAGGCTTCCATTGACCGAAGTC 3541 cagagcgcagataccaaatactgttcttctagtgtagccgtagttaggccaccacttcaa 3600 GTCTCGCGTCTATGGTTTATGACAAGAAGATCACATCGGCATCAATCCGGTGGTGAAGTT AlwNI | 3601 gaactctgtagcaccgcctacatacctcgctctgctaatcctgttaccagtggctgctgc 3660 CTTGAGACATCGTGGCGGATGTATGGAGCGAGACGATTAGGACAATGGTCACCGACGACG 3661 cagtggcgataagtcgtgtcttaccgggttggactcaagacgatagttaccggataaggc 3720 GTCACCGCTATTCAGCACAGAATGGCCCAACCTGAGTTCTGCTATCAATGGCCTATTCCG 3721 gcagcggtcgggctgaacggggggttcgtgcacacagcccagcttggagcgaacgaccta 3780 CGTCGCCAGCCCGACTTGCCCCCCAAGCACGTGTGTCGGGTCGAACCTCGCTTGCTGGAT 3781 caccgaactgagatacctacagcgtgagctatgagaaagcgccacgcttcccgaagggag 3840 GTGGCTTGACTCTATGGATGTCGCACTCGATACTCTTTCGCGGTGCGAAGGGCTTCCCTC 3841 aaaggcggacaggtatccggtaagcggcagggtcggaacaggagagcgcacgagggagct 3900 TTTCCGCCTGTCCATAGGCCATTCGCCGTCCCAGCCTTGTCCTCTCGCGTGCTCCCTCGA 3901 tccagggggaaacgcctggtatctttatagtcctgtcgggtttcgccacctctgacttga 3960 AGGTCCCCCTTTGCGGACCATAGAAATATCAGGACAGCCCAAAGCGGTGGAGACTGAACT 3961 gcgtcgatttttgtgatgctcgtcaggggggcggagcctatggaaaaacgccagcaacgc 4020 CGCAGCTAAAAACACTACGAGCAGTCCCCCCGCCTCGGATACCTTTTTGCGGTCGTTGCG 4021 ggcctttttacggttcctggccttttgctggccttttgctcacatgt 4067 CCGGAAAAATGCCAAGGACCGGAAAACGACCGGAAAACGAGTGTACA